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. 2020 Oct 13;10:17058. doi: 10.1038/s41598-020-74087-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression related to ovarian reserve in vivo. Mice at 21 days of age were treated daily with control fluid (1% carboxymethyl cellulose) or olaparib (300 mg/kg body weight) for 2 weeks before real-time RT-PCR analyses of transcript levels for various ovarian genes. (a) VEGFR-1 (p = 0.0007), (b) VEGFR-2 (p = 0.0007), (c) VEGF, (d) CD31 (p = 0.0012), (e) CYP19a (p = 0.013), (f) FSHR, (g) GDF9 (p = 0.0025), (h) Ki-67. VEGFR vascular endothelial growth factor receptor, VEGF vascular endothelial growth factor, FSHR follicle-stimulating hormone receptor, GDF9 growth differentiation factor-9. C control group, Ola olaparib group. Means ± SD of 8 control and 12 olaparib samples. *p < 0.01, **p < 0.05, significantly different between groups.