Table 2:

Distribution of animals and analyses carried out in this study.

Sample size (N)	Source of bone graft	Time of incubation after harvest (hours)	Type of analysis	Timepoint of analysis	Shown in
3	Mouse mxM1 healed site	0	TUNEL	immediately after harvest	Fig. 1B
3	Mouse mxM1 healed site	0, 1	PCNA IHC, DAPI	post-transplant day 3	Fig. 1D
5	Osseo-shaping instrument-produced osseous coagulum	0	TUNEL, DAPI, IHC, histology	post-implant day 0	Fig. 1F–H Fig. 2B
5	Handheld bone scraper-produced bone chips	0	Histology, DAPI	post-implant day 0 post-implant day 7	Fig. 2A Fig. 2C,E
5	Osseo-shaping instrument-produced osseous coagulum	0	IHC	post-implant day 3	Fig. 2G–I
5	Osseo-shaping instrument-produced osseous coagulum	0	histology, DAPI, IHC, TRAP, ALP	post-implant day 7	Fig. 2D,F,J–L
3	osseous coagulum	0	IHC	post-transplant day 0 post-transplant day 7	Fig. 2M Fig. 2N–P
Sample size (N)	Site	rodent species	Type of analysis	Timepoint of analysis	Shown in
>20	intact PDL	rat, mouse	histology	Intact state	Fig. 3A, B
>20	fresh extraction socket	rat, mouse	histology	Intact state	Fig. 3C,D
>10	extraction socket gap area around an immediate implant	rat	histology, IHC, ALP	post-implant day 0	Fig. 3F,F’
>10	extraction socket gap area around an immediate implant	mouse	IHC	post-implant day 7	Fig. 3G,M
>10	extraction socket gap area around an immediate implant	rat	ALP, μCT	post-implant day 0	Fig. 3H,K,L
3	extraction socket gap area around an immediate implant +L-PBS	rat	IHC, μCT, histology	post-implant day 3, post-implant day 14	Fig. 4A,C,E,F,I
4	extraction socket gap area around an immediate implant +L-WNT3A	rat	IHC, μCT, histology	post-implant day 3, post-implant day 14	Fig. 4B,D,G,H,J