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. Author manuscript; available in PMC: 2021 Oct 9.
Published in final edited form as: Circ Res. 2020 Jul 31;127(9):1122–1137. doi: 10.1161/CIRCRESAHA.119.316267

Figure 4. ALK1-OE restores aberrant SMC coverage observed in Eng-iKO retinal vasculature.

Figure 4.

A-F, ENG (red) and IB4 (blue) fluorescence staining with retinas isolated from PN7 control (SclCreER-negative Eng-iKO, A and D), SclCreER;Eng-iKO (B and E), and SclCreER;Eng-iKO;ALK1-OE (C and F) mice. G-I. Double staining of IB4 (blue) and SMA (red) in PN7 retinas of controls (G), SclCreER;Eng-iKO (H), and SclCreER;Eng-iKO;ALK1-OE (I) mice. GFP reporter expression in IB4-positive retinal ECs in SclCreER;Eng-iKO;ALK1-OE (F and I). Yellow arrowheads indicate developed retinal AVMs. a, artery; v, vein. Scale bars: 100 μm.