Skip to main content
. Author manuscript; available in PMC: 2021 Oct 9.
Published in final edited form as: Circ Res. 2020 Jul 31;127(9):1122–1137. doi: 10.1161/CIRCRESAHA.119.316267

Figure 5. Disrupted arteriovenous EC identities in Eng-deficient vasculature are rescued by ALK1-OE.

Figure 5.

A-F, Immunostaining on EMCN (red) and IB4 (blue) in PN 7 retinas of control (SclCreER-negative Eng-iKO, A and D), SclCreER;Eng-iKO (B and E), and SclCreER;Eng-iKO;ALK1-OE (C and F) mice. Merged images of EMCN, GFP, and IB4 (D-F). G-L, JAG1 (red) expression in PN7 retinas of controls (G and J), SclCreER;Eng-iKO (H and K), and SclCreER;Eng-iKO;ALK1-OE (I and L) mice. ECs are marked with IB4 (blue) staining. GFP signals in IB4-stained retinal ECs (J-L). Yellow arrowheads indicate AVMs in the retinal vasculature. a, artery; v, vein. Scale bars: 100 μm.