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. Author manuscript; available in PMC: 2021 Oct 9.
Published in final edited form as: Circ Res. 2020 Jul 31;127(9):1122–1137. doi: 10.1161/CIRCRESAHA.119.316267

Figure 6. Altered NOTCH and SMAD downstream genes in Eng-iKO ECs are restored by ALK1-OE.

Figure 6.

Quantitative RT-PCR analysis of Eng (A) and Alk1 (B), and Id1 (C)and NOTCH downstream genes [Hey1 (D), Jag1 (E), and Jag2 (F)] in retinal ECs purified from control (SclCreER-negative Eng-iKO, n=6), SclCreER;Eng-iKO (n=6), and SclCreER;Eng-iKO;ALK1-OE (n=6) mice. The mRNA levels of the genes are normalized with actin. One-way ANOVA with Tukey’s correction. All data are means ± SDs.