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. Author manuscript; available in PMC: 2020 Oct 14.
Published in final edited form as: J Med Chem. 2020 Sep 18;63(19):10855–10878. doi: 10.1021/acs.jmedchem.0c00545

Figure 10.

Figure 10.

Immunofluorescent staining: The DPAGT1 Expression Level in the Selected Cancer Cell Lines (PD002 and PANC-1) and a Colorectal Adenocarcinoma (HCT-116) Treated with CPPB.a

aFluorescence microscopy images at 20x. The cells (1 ×105–6) were treated with CPPB (0.05, 0.2, 2.0, and 20 μM) or PBS for 72h. The cells were treated with DPAGT1 polyclonal antibody (Invitrogen, PA5–72704), followed by secondary antibody, Donkey anti-rabbit IgG (H+L), Alexa Fluor™ 555 (red) (Invitrogen). DAPI (4′,6-diamidino-2-phenylindole), a blue fluorescent DNA dye, was used to mark the nucleus.