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. Author manuscript; available in PMC: 2020 Oct 14.
Published in final edited form as: J Med Chem. 2020 Sep 18;63(19):10855–10878. doi: 10.1021/acs.jmedchem.0c00545

Figure 7.

Figure 7.

Immuno-fluorescent staining: Effect of a DPAGT1 Inhibitor, CPPB, on Snail in Pancreatic Cancer Cells (PD002 and PANC-1) and a Colorectal Adenocarcinoma (HCT-116). Western Blotting Assay for a Cervical Cancer (SiHa).a

aFluorescence microscopy images at 40x. The cells (1 ×105–6) were treated with CPPB (0.05, 0.2, 2.0, and 20 μM) or PBS for 24h. For immunefluorescent studies: the cells were treated with Snail (C15D3) rabbit mAb (Cell Signaling Technology), followed by Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™568 (red). DAPI (4′,6-diamidino-2-phenylindole), a blue fluorescent DNA dye, was used to mark the nucleus. 40x. For Western blotting assays: The cells were treated with Snail (C15D3) rabbit mAb (Cell Signaling Technology), followed by secondary antibody, horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology).