Figure 8.

Immunofluorescent Staining: Effect of a DPAGT1 inhibitor, CPPB, on E-Cadherin in Pancreatic cancer cells (PD002 and PANC-1) and a Colorectal Adenocarcinoma (HCT-116).^a

^aFluorescence microscopy images at 20x. The cells (1 ×10^5–6) were treated with CPPB (0.05, 0.2, 2.0, and 20 μM) or PBS for 24h. The cells were treated with E-cadherin (4A2) mouse mAb (Cell Signaling Technology), followed by secondary antibody, goat anti-mouse IgG (H+L), Alexa Fluor™ Plus 647 (Invitrogen). DAPI (4′,6-diamidino-2-phenylindole), a blue fluorescent DNA dye, was used to mark the nucleus. 40x