. Author manuscript; available in PMC: 2021 Feb 10.

Published in final edited form as: Nat Struct Mol Biol. 2020 Aug 10;27(10):875–885. doi: 10.1038/s41594-020-0472-y

Table 2.

Cryo-EM data collection, refinement and validation statistics

	ZIKV NS5 – hSTAT2 (EMD-21618, PDB 6WCZ)
Data collection and processing
Magnification	x130,000
Voltage (kV)	300
Electron exposure (e^–/Å²)	48
Defocus range (μm)	1.0 – 3.0
Pixel size (Å)	1.07
Symmetry imposed	C1
Initial particle images (no.)	868,048
Final particle images (no.)	118,760
Map resolution (Å)	4.0
FSC threshold	0.143
Map resolution range (Å)	7.0 – 3.5

Refinement
Initial model used	6UX2
Model resolution (Å)	4.0
FSC threshold	0.143
Model resolution range (Å)	274 – 4.0
Map sharpening B factor (Å²)	−167
Model composition
Non-hydrogen atoms	9,893
Protein residues	1,231
Ion (zinc)	2
B factors (Å²)
Protein	153.77
Ion (zinc)	106.22
R.m.s. deviations
Bond lengths (Å)	0.01
Bond angles (°)	1.01
Validation
MolProbity score	2.55
Clashscore	13.3
Poor rotamers (%)	2.08
Ramachandran plot
Favored (%)	86.98
Allowed (%)	13.02
Disallowed (%)	0