Table 2.

Video acquisition protocol by laser confocal video microscopy.

1	Attach the VivaScope 1500 to the selected skin area of interest.
2	Move to the depth with visible capillaries, typically, at the DEJ or slightly below (~50–80 μm below the skin surface).
3	Move in the x-y plane within the total FOV (8×8 mm2) to select a 0.5×0.5 mm2 area with presence of at least one capillary with (a) a clearly visible blood flow, and/or (b) adherent or rolling leukocyte.
4	Move along the z axis to select a depth where blood flow and/or an individual leukocyte is most clearly visible (blood cells are in focus).
5	Take a 30-second video.
6	Repeat steps 4–7 to collect ten 30-second videos per imaging site. Cover different areas of the total FOV.