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. Author manuscript; available in PMC: 2021 Feb 17.
Published in final edited form as: Nat Struct Mol Biol. 2020 Aug 17;27(10):901–912. doi: 10.1038/s41594-020-0475-8

Extended Data Figure 5. Representative raw data from flow cytometry.

Extended Data Figure 5.

Graphs were drawn with pseudocolor in FlowJo. a. Gating strategy to select for single, live, intact cells. Events were gated through three serial gates to obtain approximately 25000 events for downstream analysis. Total number of events in each graph, and the percentage of events within the gate in each graph are shown. (FSC: forward scatter; SSC: side scatter; A: area; H: height; W: width.) b,c. Compensated values of the three fluorophores used (dsRED, EGFP, Cerulean), in positive and control samples with (b) primary and (c) secondary motifs.