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. Author manuscript; available in PMC: 2021 Oct 9.
Published in final edited form as: Circ Res. 2020 Aug 9;127(9):e184–e209. doi: 10.1161/CIRCRESAHA.120.316704

Figure 3. Differential enhancer utilization during embryonic heart development.

Figure 3.

A. Delineation of three major stages of heart development during the embryonic period based on Carnegie staging. B. Heatmap of signal at putative enhancers differentially marked with H3K27ac C. Same as B but with H3K4me2. D.Heatmap of z-scores for level of significance of motifs enriched in each class of differentially regulated enhancers based on pairwise comparisons of replicates of H3K27ac signal at all embryonic heart enhancer segments using DiffBind. Comparisons are indicated as follows: early up versus mid (EVM), early up versus late (EVL), mid up versus early (MVE), mid up versus late (MVL), late up versus mid (LVM), late up versus early (LVE). The more significantly enriched motifs are colored yellow. E. Same as in D but using H3K4me2 signals. F. Heatmap of most variable z-scores for significance of enrichment of gene ontology categories for genes assigned a differentially activated enhancer by GREAT.