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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Virology. 2020 Aug 22;550:51–60. doi: 10.1016/j.virol.2020.08.003

Figure 2. Expression of chimeric NAe-HA and M2e-HA proteins in recombinant attenuated A/PR8 viruses.

Figure 2.

The expression of chimeric NAe-HA and M2e-HA proteins in recombinant A/PR8 viruses was determined by Western blot and ELISA. HCA-2 or 14C2 mAbs specific for NA222–230 (NAe) and M2e epitope respectively were used to probe chimeric NAe-HA and M2e-HA proteins. Mouse immune sera collected from mice infected with sublethal A/PR8 virus were used to prove A/PR8 HA proteins. (A) Western blot analysis. Lane 1: NAe-HA A/PR8 virus (10 μg), Lane 2: M2e-HA A/PR8 virus (10 μg), Lane 3: WT-HA A/PR8 virus (10 μg). Red color arrows indicate chimeric HA proteins with NAe or M2e, and WT-HA. (B-D) The reactivity of recombinant PR8 viruses to specific HCA-2 and 14C2 mAbs or polyclonal antibodies was determined by ELISA. (B) The reactivity of recombinant PR8 viruses to NA222–230 specific HCA-2 mAb. (C) The reactivity of recombinant PR8 viruses to M2e specific 14C2 mAb. (D) The reactivity of recombinant PR8 viruses to A/PR8 polyclonal anti-sera.