. Author manuscript; available in PMC: 2021 Nov 1.

Published in final edited form as: Food Chem Toxicol. 2020 Aug 22;145:111695. doi: 10.1016/j.fct.2020.111695

Table 1.

Internal concentrations of PFOS and PFBS after treatments.

		Dose (μM)	PFOS (ng/mg DNA)	PFBS (ng/mg DNA)
Worms	Control	0	N.D	N.D

	PFOS	10	174.4 ± 9.6^b	N.D
		20	238.2 ± 5.0^a	N.D
		40	223.1 ± 7.3^a	N.D

	PFBS	1,000	N.D	2.8 ± 0.1^b
	PFBS	2,000	N.D	4.6 ± 0.2^a

Embryos	Control	0	N.D	N.D

	PFOS	10	28.5 ± 0.4^a	N.D
		20	30.5 ± 1.1^a	N.D
		40	23.6 ± 0.8^b	N.D

	PFBS	1,000	N.D	1.8 ± 0.1^b
	PFBS	2,000	N.D	3.3 ± 0.2^a

Synchronized L4/young adult worms were treated with control (0.1% DMSO) or PFOS or PFBS for 1 day at 20°C, then harvested for the determination of internal concentrations of PFOS and PFBS in worms (n=3 plates, each plate contained >1,000 nematodes). For the determination of internal concentrations of PFOS and PFBS in embryos, synchronized L4/young adult worms (F0 generation) were treated with control (0.1% DMSO) or PFOS or PFBS for 2 day at 20°C (n=3 plates, each plate contained >5,000 nematodes), then eggs were collected by the bleaching method. PFOS and PFBS concentration in worms and embryos were measured by LC/MS and normalized by DNA level. Results are expressed as mean±S.E. (n=3).

^a,b

Means with different letters are significantly different (P<0.05). N.D: not detected