Figure 2.

β-catenin regulates metabolic pathways in adipocytes and exclusively mediates effects of canonical Wnt3a signaling. RNA-seq analyses were performed on β-cat^fl/fl and β-cat^−/− adipocytes under basal conditions or after 4 h of treatment with recombinant Wnt3a (20 ng/ml; n = 4 per group). (A) Heat maps of differential gene expression changes in β-cat^fl/fl and β-cat^−/− adipocytes under basal conditions (left panel) and β-cat^fl/fl cells treated with vehicle or Wnt3a (right panel). (B) Gene Set Enrichment Analyses (GSEA) of genes expressed in β-cat^fl/fl and β-cat^−/− adipocytes under basal conditions (top panel) and β-cat^fl/fl cells treated with vehicle or Wnt3a (bottom panel). (C-D) MA plots of gene expression changes following Wnt3a treatment of β-cat^fl/fl or β-cat^−/− adipocytes. (E) Venn diagram depicting meta-analysis of gene expression changes in β-cat^fl/fl or β-cat^−/− adipocytes treated with vehicle or Wnt3a for 4 h. (F) Expression of Ctnnb1 and downstream Wnt target genes in β-cat^fl/fl and β-cat^−/− adipocytes treated with vehicle or 20 ng/ml recombinant Wnt3a for 4, 12, or 24 h (n = 6). RNA expression normalized to PPIA. Data presented as mean ± S.D. ∗ indicates significance at p < 0.05.