Figure 5.

β-catenin is up-regulated in the stromal-vascular fraction of adipose tissues from knockout mice. (A) Genetic recombination in tissues isolated from β-cat^fl/fl and β-cat^−/− mice (n = 3). (B–C) Ctnnb1 mRNA (n = 3) and protein (n = 6) expression in eWAT and iWAT of β-cat^fl/fl and β-cat^−/− mice. (D) Genomic recombination of β-catenin in adipocytes and SVF isolated from eWAT and iWAT of β-cat^fl/fl and β-cat^−/− mice. (E) Ctnnb1 mRNA expression in isolated eWAT adipocytes and SVF of β-cat^fl/fl and β-cat^−/− mice (n = 6). (F) β-catenin protein expression in isolated eWAT adipocytes and SVF of β-cat^fl/fl and β-cat^−/− mice; adiponectin and laminin shown as protein loading controls. (G-H) Wnt target gene expression in adipocytes and SVF isolated from eWAT of β-cat^fl/fl and β-cat^−/− mice (n = 8). (I) Representative plots showing flow cytometry analysis of SVF isolated from β-cat^fl/fl and β-cat^−/− mice (3 mice per sample; n = 3 samples). (J) Quantification of SVF cell proportions evaluated by flow cytometry analysis (3 mice per sample; n = 3 samples). (K) Ctnnb1 mRNA expression normalized to PPIA in cellular fractions isolated by FACS analysis (3 mice per sample; n = 3 samples). Data presented as mean ± S.D. ∗ indicates significance at p < 0.05.