FIGURE 1.

ASC-CM factors preserving transendothelial permeability partition with MW > 50 kDa. HPAEC grown on gold electrodes of ECIS arrays were exposed to (A) the original growth media (black, red) or ASC-CM (gray, green), (B) 50 kDa flow-through fractions of the growth media (black, red) or of ASC-CM (gray, green), and (C) 100 kDa flow-through fractions of the growth media (black, red) or of ASC-CM (gray, green). Media were removed after 72 h of pretreatment, and HPAEC were challenged with 250 μM H₂O₂ (red, green) or vehicle (black, gray). Data are presented as mean ± SEM from three parallel recordings; the results were reproduced in at least three independent experiments. ^∗Repeated measurement one-way ANOVA detected significant differences in (A) responses of HPAEC exposed to unmanipulated ASC-CM (green) when compared to unmanipulated growth media (red) and (C) responses of HPAEC exposed to 100 kDa flow-through of ASC-CM (green) when compared to 100 kDa flow-through of growth media (red).