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. 2020 Sep 30;8:584653. doi: 10.3389/fcell.2020.584653

FIGURE 11.

FIGURE 11

Inhibition of JNK activation is mediated by small MW factors in ASC-CM which are not species-specific. Depletion of heme oxygenase 1 in ASC attenuates ASC-CM ability to inhibit JNK activation. (A) HPAEC pretreated with the original growth media and ASC-CM or the 3 kDa flow-through fractions of growth media and ASC-CM were challenged with 2 ng/ml TNFα for 4 h. (B) HPAEC pretreated with growth media or rat ASC-CM were challenged with 2 ng/ml TNFα for 4 h. (C) HPAEC pretreated with growth media or ASC-CM generated by non-specific RNA-treated ASC (NS RNA ASC-CM) or heme oxygenase 1 siRNA-treated ASC (siRNA ASC-CM) were challenged with 2 ng/ml TNFα for 4 h. Cell lysates were analyzed with antibodies to p-JNK and β-actin (loading control). Data from three independent experiments were pooled and presented as cleaved caspase 3/β-actin ratio mean ± SEM. ∗Significance between TNF-stimulated groups assessed by t-test with Welch’s correction as indicated (p < 0.05).