FIGURE 5.

ASC-CM factors protecting from barrier dysfunction are species-specific, whereas anti-apoptotic factors are not. HPAEC (A) or RLEC (B) grown on gold electrodes of ECIS arrays were exposed to the original growth media (black, red), human ASC-CM (gray, green), or rat ASC-CM (brown, orange). Media were removed after 72 h of endothelial cell exposure, and HPAEC/RLEC were challenged with 250 μM H₂O₂ (red, green, orange) or vehicle (black, gray, brown). Shown are the means ± SEM of three parallel recordings. (C) HPAEC were pretreated with growth media or rat ASC-CM. After 72 h, media was substituted with EBM-2; HPAEC were challenged with 2 ng/ml TNFα for 4 h. Cell lysates were analyzed with antibodies to cleaved caspase 3 and β-actin (loading control).