FIGURE 8.

Carbon monoxide protects the endothelium from apoptosis. HPAEC were exposed to the original growth media or growth media supplemented with 50 μM CORM2 (CO donor) or 50 μM NaHS (H₂S donor). After 48 h, media was removed; cells were stimulated with 2 ng/ml TNFα. Cell lysates were analyzed with antibodies to cleaved and total caspase 3. Data from three independent experiments were pooled and presented as cleaved/total caspase 3 ratio mean ± SEM. One-way ANOVA with Tukey post hoc was used to detect whether differences between indicated columns are significant ^∗(p < 0.05) or not significant (NS).