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. Author manuscript; available in PMC: 2021 Oct 7.
Published in final edited form as: Cell Host Microbe. 2020 Aug 4;28(4):534–547.e3. doi: 10.1016/j.chom.2020.06.019

Figure 3. Susceptibility of P. aeruginosa CF isolates to the BcAU1054 T6SS correlates with patient age.

Figure 3.

(A) Competition experiments between WT and Δhcp BcAU1054 inhibitor strains and WT and vipA1::Tn PAO1 target strains. (B) Competition experiments between WT and Δhcp BcAU1054 inhibitor strains and P. aeruginosa infant/child CF isolate targets. (C) Immunoblot for Hcp1 production by PAO1 and P. aeruginosa infant/child CF isolates. (D) Competition experiments between WT and Δhcp BcAU1054 inhibitor strains and P. aeruginosa teenage/adult CF isolate targets. (E) Immunoblot for Hcp1 production by PAO1 and P. aeruginosa teenage/adult CF isolates. For (A, B, and D), circles/diamonds represent individual cocultures from two biological replicates, each with four technical replicates. Grey-filled diamonds represent competitions from which no target cells were recovered. Solid horizontal lines represent mean log10 C.I. values. Dotted horizontal lines (log10 C.I. = 0) indicate no competitive advantage for either strain. *P<0.05, **P<0.005, ***P<0.0005, Mann-Whitney test. For (C and E), non-specific band above Hcp1 serves as loading control. Blots are representative of at least two experiments per strain.