KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-PAO1 Hcp1 polyclonal peptide antibody	Gift from Dr. John Mekalanos	N/A
IRDye® 800CW Goat anti-rabbit IgG Secondary Antibody	LI-COR Biosciences	926–32211; RRID: AB_621843
Bacterial and Virus Strains
All bacterial strains used in this study listed in Table S3
Chemicals, Peptides, and Recombinant Proteins
Gentamicin	Gold Biotechnology	G-400–25
Kanamycin	Gold Biotechnology	K-120–25
Nalidixic acid	Sigma-Aldrich	N4382
Trimethoprim	Gold Biotechnology	T-350–5
Tetracycline	Fisher Scientific	BP912–100
Chloramphenicol	Fisher Scientific	BP904–100
5-bromo-4-chloro-3-indoxyl-β-D-glucuronide (X-Gluc)	Gold Biotechnology	G1281C1
5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal)	Gold Biotechnology	X4281C
L-arabinose	Gold Biotechnology	A-300–100
D-sucrose	Fisher Scientific	BP220–1
2,6-diaminopimelic acid	Alfa Aesar	AAB22391–06
Critical Commercial Assays
Wizard Genomic DNA Purification Kit	Promega	A1120
GenElute™ Bacterial Genomic DNA Kit	Sigma-Aldrich	NA2110
Deposited Data
P. aeruginosa isolate whole genome sequences	This study	NCBI Sequence Read Archive: PRJNA607994 and PRJNA609958
Recombinant DNA
All plasmids used in this study listed in Table S4
Software and Algorithms
Geneious Prime	Geneious, Software, Newark, New Jersey, USA	http://www.geneious.com; RRID: SCR_010519
Phyre2	(Kelley et al., 2015)	http://www.sbg.bio.ic.ac.uk/~phyre2; RRID: SCR_010270
HHPred	(Zimmermann et al., 2018)	https://toolkit.tuebingen.mpg.de/tools/hhpred; RRID: SCR_010276
Mauve	(Darling et al., 2004)	http://gel.ahabs.wisc.edu/mauve/; RRID: SCR_012852
GraphPad Prism version 8.0 for Mac	GraphPad, Software, La Jolla, California, USA	http://www.graphpad.com; RRID: SCR_022798