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. 2020 Sep 30;8:579629. doi: 10.3389/fcell.2020.579629

TABLE 1.

List of the human-specific primer sequences used for qPCR analyses.

Gene Primer Sequence
Tm (°C)
Forward Reverse
STAT1 5′-ttcaggaagacccaatccag-3′ 5′-tgaatattccccgactgagc-3′ 60
p53 5′-gttccgagagctgaatgagg -3′ 5′-tctgagtcaggcccttctgt-3′ 60
Bcl-2 5′-atgtgtgtggagagcgtcaa-3′ 5′-acagttccacaaaggcatcc-3′ 60
p38 5′-tgactcagatgccgaagatg-3′ 5′-atcataaggatcggccactg-3′ 60
p21 5′-ggaagaccatgtggacctgt-3′ 5′-ggattagggcttcctcttgg-3′ 60
MDM2 5′- ggtgggagtgatcaaaagga-3′ 5′-gtggcgttttctttgtcgtt-3′ 60
CDK4 5′-cccgaagttcttctgcagtc-3′ 5′-ctggtcggcttcagagtttc-3′ 60
Cyclin D1 5′- cctgtcctactaccgcctca -3′ 5′- cacctcctcctcctcctctt -3′ 60
IL-6 5′-actcacctcttcagaacgaattg-3′ 5′-ccatctttggaaggttcaggttg-3′ 60
IL-8 5′-actgagagtgattgagagtggac-3′ 5′-aaccctctgcacccagttttc-3′ 60
GLUT1 5′-gcgggttgtgccatactcatgacc-3′ 5′-aggccacaaagccaaagatggcc-3′ 59
HK II 5′-gagccaccactcaccctact-3′ 5′-ccaggcattcggcaatgtg-3′ 60
LDHA 5′-ttgacctacgtggcttggaag-3′ 5′-ggtaacggaatcgggctgaat-3′ 60
β-tubulin 5′-aagatccgagaagaataccctga-3′ 5′-ctaccaactgatggacggaga-3′ 60

STAT1, signal transducer and activator of transcription 1; Bcl-2, B-cell lymphoma 2; CDK4, cyclin dependent kinase 4; IL-6, interleukin 6; IL-8, interleukin 8; Glut1, glucose transporter 1; LDHA, lactate dehydrogenase A.