TABLE 1.
Gene |
Primer Sequence |
Tm (°C) | |
Forward | Reverse | ||
STAT1 | 5′-ttcaggaagacccaatccag-3′ | 5′-tgaatattccccgactgagc-3′ | 60 |
p53 | 5′-gttccgagagctgaatgagg -3′ | 5′-tctgagtcaggcccttctgt-3′ | 60 |
Bcl-2 | 5′-atgtgtgtggagagcgtcaa-3′ | 5′-acagttccacaaaggcatcc-3′ | 60 |
p38 | 5′-tgactcagatgccgaagatg-3′ | 5′-atcataaggatcggccactg-3′ | 60 |
p21 | 5′-ggaagaccatgtggacctgt-3′ | 5′-ggattagggcttcctcttgg-3′ | 60 |
MDM2 | 5′- ggtgggagtgatcaaaagga-3′ | 5′-gtggcgttttctttgtcgtt-3′ | 60 |
CDK4 | 5′-cccgaagttcttctgcagtc-3′ | 5′-ctggtcggcttcagagtttc-3′ | 60 |
Cyclin D1 | 5′- cctgtcctactaccgcctca -3′ | 5′- cacctcctcctcctcctctt -3′ | 60 |
IL-6 | 5′-actcacctcttcagaacgaattg-3′ | 5′-ccatctttggaaggttcaggttg-3′ | 60 |
IL-8 | 5′-actgagagtgattgagagtggac-3′ | 5′-aaccctctgcacccagttttc-3′ | 60 |
GLUT1 | 5′-gcgggttgtgccatactcatgacc-3′ | 5′-aggccacaaagccaaagatggcc-3′ | 59 |
HK II | 5′-gagccaccactcaccctact-3′ | 5′-ccaggcattcggcaatgtg-3′ | 60 |
LDHA | 5′-ttgacctacgtggcttggaag-3′ | 5′-ggtaacggaatcgggctgaat-3′ | 60 |
β-tubulin | 5′-aagatccgagaagaataccctga-3′ | 5′-ctaccaactgatggacggaga-3′ | 60 |
STAT1, signal transducer and activator of transcription 1; Bcl-2, B-cell lymphoma 2; CDK4, cyclin dependent kinase 4; IL-6, interleukin 6; IL-8, interleukin 8; Glut1, glucose transporter 1; LDHA, lactate dehydrogenase A.