Figure 3.

HotairM1 Depletion Facilitates Tumor Cell Proliferation and Migration

(A) The proliferation ability of HotairM1-knockdown and shCtrl tumor cells was analyzed using the CCK8 assay. The optical density 450 nm (OD₄₅₀) value was recorded, and the growth index was analyzed. (B) Images of colonies formed from the plate cloning assay are shown. (C) Colonies formed in the well were calculated and analyzed. Data are indicated as the mean ± SD. (D) Tumorigenesis ability was determined using a soft agar assay. Representative images of stained colonies formed in the upper agar are shown. (E) The colony number in the upper agar was counted, and the relative colonies per site were calculated. Data are indicated as the means ± SD. (F) Migration ability of shHotairM1 and shCtrl tumor cells was analyzed through a Transwell assay. Images of cells on the outside of the Transwell are shown. (G) The OD₆₃₀ absorbance values of stained migrated cells were obtained to calculate the migration rate. Data are indicated as the mean ± SD. ∗p < 0.05; ∗∗p < 0.01, by two-tailed Student’s t test.