Figure 5.

bHDACi Differentially Regulates NIS Expression across Organs In Vivo

(A) Photomicrographs of NIS IHC performed on mouse thyroid, lung, ovary, stomach, and liver tissues harvested from untreated control or MS-275-treated mouse. DAB staining (brown) and respective H&E-stained images are displayed. (B) Histogram profiles of DAB-stained tissue sections showing NIS intensity profile obtained by using IHC Profiler program. (C) Images of gel picture showing amplified NIS transcripts as a measure of response to VPA and MS-275 drug treatment in various cancer cell lines, i.e., BC (ZR-75-1), lung cancer (A-549), hepatocellular carcinoma (HEPG2), ovarian cancer (A2780), and glioblastoma (U87). β-actin housekeeping gene is used as loading control.