Figure 6.

miR-146b-5p Enhanced EGFR TKI-Mediated Apoptosis by Downregulating IRAK1

(A) PC9/gef-IRAK1 and PE2988-IRAK1 cells stably expressing IRAK1 were established by infecting PC9/gef or PE2988 cells with IRAK1 lentivirus particles (containing the IRAK1-encoding sequence but lacking the 3′ UTR), and IRAK1 expression was detected using qRT-PCR and immunoblotting. qRT-PCR data were presented as mean ± SD (Student’s t test: ∗∗∗p < 0.001). (B) After transfection of the miR-146b-5p mimic, the cells were treated with the vehicle of osimertinib (0.1 μM for PE2988; 0.3 μM for PE3479) for 18 h. The cleavage of caspase-3 and PARP were detected. (C) The expression levels of IL-6 and IL-8 were determined after transfection of miR-Ctl or miR-146b-5p mimics using qRT-PCR. qRT-PCR data were presented as mean ± SD (Student’s t test: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001). (D) Schematic diagram showing the role of miR-146b-5p in regulating EGFR TKI resistance.