Figure 6.

Effects of circFLT1 and lncCCPG1 on Adipocytes Proliferation and Apoptosis

(A and B) Overexpression/knockdown efficiency of circFLT1 (A) and lncCCPG1 (B) on cell proliferation. (C and D) Cell proliferation was measured with the 5-ethynyl-20-deoxyuridine (EdU) assay of circFLT1 (C) and lncCCPG1 (D). (E and F) The cell proliferation index was measured by the cell counting kit-8 (CCK-8) assay of circFLT1 (E) and lncCCPG1 (F). (G and H) Cell phases were analyzed using flow cytometry of circFLT1 (G) and lncCCPG1 (H). (I–L) Expression of proliferation marker genes was measured with RT-qPCR of circFLT1 (I) and lncCCPG1 (K) and western blotting of circFLT1 (J) and lncCCPG1 (L). (M–P) Expression of apoptosis marker genes was measured with RT-qPCR of circFLT1 (M) and lncCCPG1 (O) and western blotting of circFLT1 (N) and lncCCPG1 (P). Values are presented as mean ± SEM for three biological replicates. Different letters (a, b) indicate significant differences: p < 0.05; ∗p < 0.05; ∗∗∗p < 0.001.