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. 2020 Sep 30;11:559589. doi: 10.3389/fimmu.2020.559589

Figure 1.

Figure 1

ssON blocked mast cell degranulation induced by C48/80 and LL-37. Degranulation of LAD2 cells was measured by the upregulation of CD63 on the cell surface and histamine and PGD2 release. (A–C) LAD2 cells were stimulated with C48/80 (1 μM), LL-37 (10 μg/mL), SP (0.5 μM), or A23187 (2 μM) with and without ssON (0.5 μM) for 30 min. ssON significantly blocked C48/80- and LL-37-induced degranulation as shown by the reduced (A) CD63 frequency, (B) histamine release, and (C) PGD2 release, but had no effect on cells stimulated with SP or A23187. (D,E) The ssON-mediated inhibition of C48/80-induced degranulation in LAD2 cells was concentration-dependent (EC50 around 0.125 μM). Statistical significance was assessed by non-parametric Mann-Whitney test for (A,B) and two-way ANOVA test for (D,E). P-value: ****P < 0.0001, ***P = 0.0001, **P = 0.0022, ns, non-significant. Data is shown as mean ± SEM. n.d., not detectable.