Figure 2.

ssON inhibited the activation of MRGPRX2 by C48/80 and LL-37. A stably MRGPRX2-transfected HEK293 cell line was used. Intracellular calcium (Ca²⁺) influx was determined by loading cells with Fluo-3 dye and measuring the median fluorescence intensity (MFI) during treatment. MFI curves were recorded for 30 s (sec) to obtain a baseline. The cells were then stimulated during the gap in the analysis with (A,B) C48/80 or (C,D) LL-37 with and without ssON, and further recorded for 2.5 min. The Ca²⁺ influx induced by the activation of MRGPRX2 with C48/80 and LL-37 was completely blocked by ssON. Each curve represents an independent sample. Gaussian smoothing is applied. Three independent experiments were performed in duplicate. A representative result is shown.