Skip to main content
. 2020 Sep 30;11:559589. doi: 10.3389/fimmu.2020.559589

Figure 3.

Figure 3

C48/80-induced scratch behavior was inhibited by ssON in a concentration dependent manner, while PAR2-mediated scratch behavior was unaffected. (A–C) Duration of scratch behavior (seconds), (D–F) frequency of scratch behaviors, and (G–I) the mean length per scratch behavior (duration/frequency) were recorded for 1 h immediately following treatment in two separately performed experiments (A,C,D,F,G,I and B,E,H, respectively). Histamine-related scratch behavior was induced by intradermal injection of 50 μL of either C48/80 (10 μg) alone or a combination of C48/80 (10 μg) and ssON (25 μg or 2.5 μg). Non-histamine-related scratch behavior was induced by intradermal injection of 50 μL of either SLIGRL (100 μg) alone or a combination of SLIGRL (100 μg) and ssON (2.5 or 25 μg). Statistical significance was assessed by one-way ANOVA or unpaired, two-tailed t-test for (B, E,H). All data passed Shapiro-Wilk's normality test (GraphPad Prism); N = 7–9 mice. P-value: ****P < 0.0001, ***P = 0.0002–0.0005, ns, non-significant. Data is shown as mean ± SEM.