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. 2020 Sep 30;11:559589. doi: 10.3389/fimmu.2020.559589

Figure 5.

Figure 5

ssON ameliorated LL-37-mediated inflammation in a rosacea mouse model. Mice were treated subcutaneously in the skin of the back with LL-37 (320 μM), with or without ssON (57 μg), or PBS. (A) Photographs for the respective treatments were taken at the clinical endpoint, 72 h after the induction of rosacea-like inflammation. The expression of mouse chymase (Cma1), tryptase (Tpsab1), and matrix metalloproteinase-9 (Mmp9) in the skin was assessed using RT-PCR in biopsies collected (B) 1 h post treatment or (C) 72 h after observation. Representative pictures are shown for each treatment group. Statistical significance was assessed by one-way ANOVA or Kruskal-Wallis test for Tpsab1 expression in C and Mmp9 expression in B. Normality was assessed using the Shapiro-Wilk normality test (GraphPad Prism); N = 8 for (B) and N = 6 for (C). P-value: ****P < 0.0001, ***P = 0.0002–0.0006, **P = 0.0057, *P = 0.033, ns, non-significant. Data is shown as mean ± SEM. 1 h = 1 hour post treatment.