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Peptide-triggered cytoplasmic membrane depolarization of C. albicans cgmcc 2.2806. Membrane potential variations induced by different concentrations (0.5 × MIC, 1 × MIC, and 2 × MIC) of RF3 and melittin were monitored using voltage-sensitive dye 3,3′-dipropylthiadicarbocyanine (DiSC3–5). Fluorescence intensity was monitored at an excitation wavelength of 622 nm and an emission wavelength of 670 nm as a function of time.
