Fig. 1.

Trypan blue exclusion test. A: bright-field micrographs showing HepG2 before and after laser irradiation. A cell a was exposed by NIR fs laser single pulse (50 nJ/pulse, objective NA. 0.5). B: Graph showing time-dependent transmittance changes at laser irradiated cell (a), adjacent cells (b and c), bleb (d), and buffer (e). Reference time zero shows the laser irradiated period. Error bars indicate the standard deviation of intensity observed from pixels. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)