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. 2020 Sep 30;11:554520. doi: 10.3389/fpls.2020.554520

Figure 4.

Figure 4

Functional identification of SlDREBA4-silenced plants under heat-stress conditions. WT, wild-type plants; V1+V2: plants co-transformed by empty vectors pTRV1 and pTRV2; VIGS-1 to -10: SlDREBA4-silenced plants. (A) Identification of SlDREBA4-silenced plants, PDS, phenotypes of the plants with silenced PDS, RT-qPCR: transcription level of SlDREBA4 using RT-qPCR in which the level of WT was assigned a value of 1. Error bar on each column represents the SD of the three biological replicates (Student’s t-test, **P < 0.01). Silenced plants under normal (B) and heat-stress (C) conditions were grown in nutrient solution. The numbers in brackets indicate the HII values (%), which are shown as the means of the three biological replicates ± SDs (Student’s t-test, **P < 0.01).