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. 2020 Sep 30;11:554520. doi: 10.3389/fpls.2020.554520

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Copyright © 2020 Mao, Deng, Jiang, Zhu, Yang, Yue and Zhao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of DRE binding specificity of SlDREBA4. (A) Construction of the required vectors for the Y1H assay. The bait plasmid pHis2.1 contained three tandem repeat DREs or mDREs and the HIS reporter gene. The coding sequence of SlDREBA4 was cloned into the activation domain GAL4 of the prey plasmid pGADT7. (B) Yeast strain Y187 was co-transformed with bait (pHis2.1-3DRE or pHis2.1-3mDRE) and prey (pGADT7 or pGADT7-SlDREBA4) construct. Interaction between the bait and prey was examined by cell growth on SD medium lacking Trp, Leu, and His (SD-WLH) containing 3-AT.