Table 1.
Control | Heat stress | |||||||
---|---|---|---|---|---|---|---|---|
EV | L-3 | L-8 | L-12 | EV | L-3 | L-8 | L-12 | |
Proline (ng/g·FW) | 85.2 ± 6.2 | 132.8 ± 11.9** | 142.3 ± 13.9** | 156.6 ± 15.8** | 178.8 ± 10.5 | 458.6 ± 40.5** | 487.8 ± 25.9** | 603.6 ± 36.8** |
Malondialdehyde (nmol/g·FW) | 83.9 ± 7.2 | 76.9 ± 6.4 | 80.5 ± 7.8 | 75.9 ± 8.6 | 344.9 ± 23.6 | 189.3 ± 15.3** | 145.9 ± 16.9** | 159.8 ± 18.9** |
Soluble total sugar (mg/g·FW) | 21.4 ± 1.5 | 39.6 ± 2.1** | 30.9 ± 1.8** | 38.81 ± 1.0** | 42.8 ± 5.2 | 78.9 ± 6.3** | 91.6 ± 4.6** | 82.3 ± 3.6** |
Soluble starch (mg/g·FW) | 21.7 ± 1.3 | 26.3 ± 2.3* | 29.6 ± 3.1* | 28.7 ± 1.5* | 45.6 ± 1.2 | 82.6 ± 5.3** | 75.1 ± 3.6** | 91.2 ± 2.9** |
Electrolyte leakage rate (%) | 16.8 ± 0.9 | 15.6 ± 1.6 | 16.7 ± 0.8 | 15.4 ± 1.2 | 82.8 ± 9.6 | 36.1 ± 4.3** | 35.4 ± 4.1** | 38.9 ± 1.6** |
POD (U/g·FW) | 1.8 ± 0.1 | 5.7 ± 0.1** | 6.6 ± 0.2** | 4.6 ± 0.1** | 5.4 ± 0.1 | 20.1 ± 0.5** | 25.8 ± 0.3** | 30.8 ± 0.4** |
SOD (U/g·FW) | 1654.6 ± 78.9 | 3335.8 ± 121.8** | 3708.6 ± 198.6** | 3658.3 ± 154.6** | 2269.6 ± 45.5 | 6366.5 ± 69.5** | 6432.5 ± 157.6** | 8239.6 ± 110.6** |
CAT (U/g·FW) | 504.4 ± 56.4 | 935.1 ± 35.9** | 919.2 ± 23.6** | 990.9 ± 32.5** | 909.8 ± 90.6 | 2023.9 ± 58.6** | 2056.8 ± 115.6** | 2123.8 ± 69.3** |
ABA (ng/g·FW) | 44.7 ± 1.5 | 49.2 ± 5.8 | 48.9 ± 6.7 | 48.1 ± 5.2 | 127.9 ± 5.9 | 123.8 ± 9.1 | 117.7 ± 6.8 | 122.8 ± 8.3 |
JA (ng/g·FW) | 17.2 ± 2.1 | 81.3 ± 7.1** | 91.6 ± 3.9** | 87.5 ± 9.5** | 38.8 ± 2.3 | 245.6 ± 22.6** | 278.6 ± 30.2** | 215.9 ± 11.9** |
SA (ng/g·FW) | 5.5 ± 0.2 | 39.7 ± 3.6** | 45.3 ± 6.4** | 36.8 ± 1.5** | 10.6 ± 0.9 | 156.8 ± 14.5** | 145.6 ± 12.6** | 158.5 ± 10.3** |
ACC (ng/g·FW) | 756.6 ± 56.9 | 2063.8 ± 235.6** | 2653.8 ± 214.6** | 2145.8 ± 98.6** | 1231.5 ± 156.3 | 3684.1 ± 312.5** | 4123.8 ± 396.5** | 3569.8 ± 212.3** |
IAA (ng/g·FW) | 0.79 ± 0.06 | 0.2 ± 0.01** | 0.16 ± 0.01** | 0.18 ± 0.01** | 0.46 ± 0.05 | 0.1 ± 0.01** | 0.13 ± 0.01** | 0.15 ± 0.01** |
EV, empty vector-transformed lines; L-3, L-8, and L-12, SlDREBA4-overexpression lines. The results are indicated as the means of the three biological replicates ± SDs (Student’s t-test, *P < 0.05, **P < 0.01).