. 2020 Sep 30;11:554520. doi: 10.3389/fpls.2020.554520

Table 2.

Physiological indicators in SlDREBA4-silenced plants.

	Control				Heat stress
	V1+V2	VIGS-2	VIGS-4	VIGS-8	V1+V2	VIGS-2	VIGS-4	VIGS-8
Proline (ng/g·FW)	79.8 ± 3.3	69.3 ± 5.0^*	64.2 ± 7.5^*	65.4 ± 5.6^*	167.5 ± 13.8	120.8 ± 8.9^**	113.7 ± 9.0^**	119.8 ± 8.8^**
Malondialdehyde (nmol/g·FW)	78.7 ± 6.9	76.0 ± 5.6	81.6 ± 6.3	79.0 ± 6.9	289.8 ± 12.9	303.3 ± 23.8	295.8 ± 20.1	318.3 ± 30.8
Soluble total sugar (mg/g·FW)	19.0 ± 0.9	19.4 ± 1.2	20.6 ± 2.0	18.9 ± 1.6	36.9 ± 4.1	37.0 ± 4.2	32.9 ± 1.4	31.9 ± 2.7
Soluble starch (mg/g·FW)	32.1 ± 2.9	35.8 ± 2.0	31.5 ± 2.9	36.0 ± 4.4	59.3 ± 4.3	55.9 ± 4.6	56.0 ± 6.5	50.9 ± 5.8
Electrolyte leakage rate (%)	23.9 ± 1.8	24.5 ± 0.9	21.8 ± 2.1	24.5 ± 3.9	68.0 ± 3.1	81.0 ± 6.6^*	79.0 ± 9.2^*	76.8 ± 7.9^*
POD (U/g·FW)	2.3 ± 1.2	1.6 ± 0.1^*	1.6 ± 0.1^*	1.8 ± 0.02^*	7.8 ± 4.5	5.2 ± 0.4^**	4.8 ± 0.1^**	5.1 ± 0.5^**
SOD (U/g·FW)	1465.6 ± 67.8	1254.8 ± 89.7^*	1189.7 ± 123.7^*	1134.7 ± 134.7^*	3078.9 ± 445.8	2078.4 ± 312.7^**	2134.5 ± 178.9^**	2218.5 ± 221.7^**
CAT (U/g·FW)	476.8 ± 34.5	465.7 ± 34.6	509.4 ± 56.7	489.7 ± 34.6	789.9 ± 87.3	800.3 ± 39.7	768.7 ± 56.7	813.8 ± 70.4
ABA (ng/g·FW)	35.8 ± 4.3	38.6 ± 2.9	40.1 ± 4.2	36.8 ± 4.1	81.4 ± 4.0	76.5 ± 4.3	83.6 ± 7.9	74.5 ± 6.9
JA (ng/g·FW)	21.9 ± 1.5	8.8 ± 0.5^**	7.3 ± 0.9^**	8.3 ± 0.6^**	58.9 ± 4.9	25.3 ± 1.3^**	25.6 ± 1.6^**	24.6 ± 1.9^**
SA (ng/g·FW)	9.7 ± 0.2	6.6 ± 0.6^**	5.9 ± 0.6^**	4.9 ± 0.3^**	28.9 ± 1.3	10.6 ± 1.3^**	11.8 ± 1.2^**	8.4 ± 0.7^**
ACC (ng/g·FW)	1091.0 ± 114.3	265.2 ± 23.2^**	273.6 ± 25.6^**	337.5 ± 28.3^**	2584.2 ± 22.3	568.2 ± 33.6^**	568.5 ± 69.6^**	756.3 ± 53.6^**
IAA (ng/g·FW)	0.69 ± 0.05	0.73 ± 0.04	0.71 ± 0.07	0.71 ± 0.04	0.47 ± 0.03	0.51 ± 0.05	0.53 ± 0.0	0.48 ± 0.04

V1+V2: plants co-transformed by empty vectors pTRV1 and pTRV2, VIGS-2, VIGS-4, and VIGS-8: SlDREBA4-silenced plants. The results are indicated as the means of the three biological replicates ± SDs (Student’s t-test, *P < 0.05, **P < 0.01).