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. 2020 Sep 30;8:523550. doi: 10.3389/fcell.2020.523550

FIGURE 4.

FIGURE 4

ULK1 loss suppress human cholestrogenic genes via effects on AKT-FOXO3a pathway. (A,B) Representative immunoblot and densitometry showing cellular levels of phosphorylated and total AKT and FoxO3a proteins ± Ulk1 siRNA (10 nM/48 h) in HepG2 cells. Values are means ± SD (n = 3),∗p < 0.05. (C,D) Representative Immunoblot and densitometry showing protein levels of SREBF2 and HMGCR in HepG2 ± Ulk1 alone and with FoxO3a (Cell Signaling Technology, #6302) double KD (10 nM) for 48 h of treatment. Values are means ± SD (n = 3), ∗p < 0.05 between Ulk1 KD vs. Control and #p < 0.05 between Ulk1 KD + FoxO3a KD vs. Ulk1 KD alone. (E) Representative immunoblot of HepG2 cells treated with SBI-0206965 (ULK1 inhibitor) at a dose of 10 uM for 48 h. (F) Schematic diagram showing the regulation of Mevalonate/cholesterol biosynthesis pathway upon loss of ULK1.