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. 2020 Sep 30;11:566611. doi: 10.3389/fphar.2020.566611

Figure 8.

Figure 8

Effects of TFA and FEB on autophagy-mediated macrophage polarization through AMPK-SIRT1 signaling in vitro. (A) A WB analysis of BECN1 and LC3 in RAW 264.7 cells exposed to LPS (100 ng/ml) with or without TFA (20 μg/ml) or FEB (100 nM) for 24 h. (B) A WB analysis of AMPK, and SIRT1 in RAW 264.7 cells exposed to LPS (100 ng/ml) with or without TFA (20 μg/ml) or FEB (100 nM) for 24 h. The data are expressed as the mean ± SD, (n = 3). * P < 0.05, ** P < 0.01. TFA, total flavones of Abelmoschus manihot; FEB, febuxostat; BECN1, beclin1; LPS, lipopolysaccharide; NS, not significant; WB, Western blotting.