Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 30;8:575126. doi: 10.3389/fcell.2020.575126

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Zalazar, Stival, Nicolli, De Blas, Krapf and Cesari.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Distribution of SPINK3 on capacitated and non-capacitated sperm. (A) Caudal sperm were incubated in non-capacitating (NC) or capacitating (CAP) conditions and then treated with recombinant SPINK3 (13 μM, “exogenous SPINK3”) for 15 min, washed and fixed. As a control of BSA blocking effect, a NC + BSA condition was included. SPINK3 was immunodetected as detailed in Methods. (B) Sperm recovered from the uterus of synchronized females immediately after coitus or from the male epididymis (negative control of cells that were not in contact with seminal vesicle secretions) were fixed. Then, native SPINK3 (“endogenous SPINK3”) was immunodetected. For (A,B) left panel shows representative pseudocolor images and bright fields (BF), and right panel shows percentage of positive immunoreactive cells. Scale bar = 10 μM.