FIGURE 6.

Effect of SPINK3 on hyperactivation. (A) Mouse sperm were incubated in the absence or presence of different SPINK3 concentrations in medium that supports capacitation (CAP). Hyperactivation was analyzed using a Hamilton Thorne IVOS CASA system (data represents mean ± SE, n = 3; **p < 0.01 with respect to CAP control). (B) Sperm were incubated under non-capacitating (NC) or capacitating (CAP) medium for 75 min, and 1 μM SPINK3 was added either since the beginning (0 min) or at the end (60 min) of the capacitation period (data represents mean ± S.E.M., n = 3; **p < 0.01 with respect to CAP control).