Figure 6.

α-Sarcoglycan Expression in Differentiated Myotubes

(A) Wild-type, DMD, and UTRNΔIMTR differentiated myotubes were stained with DAPI (blue), MYHC (green), and α-sarcoglycan (red). Scale bar, 200 μm. (B) α-sarcoglycan quantification in wild-type, DMD, and UTRNΔIMTR differentiated myotubes. The α-sarcoglycan intensity shown as percentage expression mean ± SEM, was calculated as median of line intensity profile (n = 20) in ImageJ and normalized with DAPI expression. Differences in α-sarcoglycan expression between individual groups were analyzed by the Kruskal-Wallis multiple comparison test. The p value is <0.0001. (C) The western blot shows α-sarcoglycan expression in wild-type, DMD, and UTRNΔIMTR myotubes. Vinculin was used as loading control.