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Apoplastic sucrose declines during P. infestans infection. Infected and mock-infected potato and tomato leaflets, after 24 h, were infiltrated with buffer. After the fluid was recovered by centrifugation, disaccharides (mostly sucrose) were quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a standard curve. Values were adjusted to the true apoplastic concentration based on an indigo carmine dilution assay.
