FIG 2.

P. infestans invertase enables P. pastoris to use sucrose as a carbon source. P. pastoris strain KM71H and a version expressing PITG_14238 (INV) were grown on minimal medium with or without sucrose (SUC), plus methanol to induce the gene. Similar results were obtained with three independent transformants of KM71H, as well as with three independent transformants of strain X33, and when cells from methanol-induced cultures were pelleted and resuspended in methanol-free medium with or without sucrose.