FIG 4.

Expression of invertase genes. (A) mRNA levels of PITG_14237 and PITG_14238 were measured using RNA-seq data from young nonsporulating hyphae grown on glucose-containing minimal medium or rye-sucrose medium, sporangia from rye-sucrose (rye-suc) medium, sporangia chilled for 60 min to stimulate zoosporogenesis (cleaving sporangia), zoospores after 60 min of swimming, germinated zoospore cysts, potato leaves at 2, 4, and 6 dpi, and tubers at 1.5, 2.5, and 4 dpi. The first time point for each type of plant tissue represents biotrophic growth, and the last time point represents the necrotrophic stage. (B) mRNA levels in nonsporulating hyphae from rye-sucrose medium and sporangia isolated from rye-sucrose cultures, potato leaves, or tubers. Per-gene normalization was performed separately in each panel to a mean of 1.0.