FIG 6.

Scanning electron microscopy of crude WT and mutant L. donovani-infected liver homogenates. Dissected mouse liver samples were crushed in the presence of Karnovsky fixative to prepare a crude liver homogenate, which was processed further with tannic acid fixation and 4% osmium tetroxide. The samples were serially dehydrated in ethanol, coated with gold, and visualized using an FEI Quanta 200 FEG electron microscope at 20 kV. (a) WT-infected liver tissue piece with amastigotes seen underneath the tissue piece. (b to d) Enlarged images from the wild-type-infected liver tissue piece. (e and f) D6-infected liver tissue piece with amastigotes found negative.