FIG 4.

GprM and GprJ affect MpkA phosphorylation. (A) Deletion strains were grown for 24 or 48 h in glucose minimal medium (GMM). (B) Overexpression strains were grown in MM before mycelia were transferred to xylose minimal medium for 30, 60, 120, and 240. Total cellular proteins were extracted and quantified, and Western blotting was run against phosphorylated MpkA (MpkA-P) using the anti-44/42 MpkA antibody. MpkA phosphorylation signals were normalized by cellular γ-actin using the anti-γ-actin antibody. Signal intensities were quantified using the ImageJ software, and ratios of MpkA-P to γ-actin were calculated.