FIG 5.

GprM and GprJ affect the cell wall organization. (A and B) Single deletion of gprM and gprJ results in increased concentrations of cell wall sugars. Strains were grown for 24 h in glucose minimal medium (GMM) before cell wall-soluble fractions were prepared and high-performance liquid chromatography (HPLC) was performed. Standard deviations present the average of six biological repetitions *, P < 0.05 in a one-way ANOVA test in comparison to the wild-type strain. (C and D) Transmission electron microscopy of hyphal germlings and conida of the ΔgprM (C) and ΔgprJ (D) strains when grown for 24 h in GMM. Shown are representative pictures of hyphal and conidia cell wall sections as well as graphs showing the average cell wall thickness (nm) of 100 sections of different hyphal germlings or conidia (average of 4 sections per germling). Standard deviations present the average of 100 measurements. *, P < 0.00001 in a one-tailed, paired t test in comparison to the wild-type (WT) strain.