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Protein kinase A (PKA) activity is reduced in the ΔgprM and ΔgprM ΔgprJ mutants. Strains were grown for 24 or 48 h in glucose minimal medium before total cellular proteins were extracted and PKA activity was measured. Standard deviations present the average of three biological replicates. **, P < 0.01 and ***, P < 0.001 (NS, nonsignificant) in a two-way ANOVA followed by Bonferroni posttests.
