FIG 8.

Functional characterization (FunCat) of significantly differently expressed genes (DEGs) identified by RNA-seq in the gprM- and gprJ-overexpressing strains. (A) FunCat analysis of DEGs up- and downregulated in the gprM-overexpressing strain in comparison to the wild-type (WT) strain when grown for 4 h in xylose minimal medium (XMM) after transfer from 24 h growth in glucose minimal medium (GMM). (B) FunCat analysis of DEGs downregulated in the gprJ-overexpressing strain in comparison to the WT strain when grown for 1 h in XMM after transfer from 24 h growth in GMM. (C) Venn diagrams showing the number of up- or downregulated DEGs specific to each deletion strain and shared among the ΔgprM and ΔgprJ strains in comparison to the WT strain (log₂FC). In parentheses is the total number of DEGs identified for each strain. A diagram depicting the growth condition used for RNA-seq is also shown.